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Part:BBa_K4850006
Rigid Protein Linker A(EAAAK)2A
A(EAAAK)2A is a specific type of rigid protein linker used for genetically fusing functional protein domains. It can maintain a fixed distance between functional domains while preserving their individual functions. This is achieved through the adoption of an alpha-helical structure, which is renowned for its rigidity and stability, attributed to the closely packed backbone structure and the presence of intra-segment hydrogen bonds. The A(EAAAK)2A protein linker was originally designed by Arai et al. (2001), and it features an empirical sequence denoted as A(EAAAK)nA, where n typically ranges from 2 to 5.
Usage and Biology
The NUS-Singapore 2023 iGEM team frequently employed this linker in their gene circuits when there was a need to connect two protein coding sequences to create a fusion protein. The part that made use of this linker include:
- BBa_K4850008: GFP-sacB Negative Selection Marker (Composite Part)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Arai, R., Ueda, H., Kitayama, A., Kamiya, N., & Nagamune, T. (2001). Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Engineering Design & Selection, 14(8), 529–532. https://doi.org/10.1093/protein/14.8.529
[2] Chen, X., Zaro, J. L., & Shen, W. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039
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